Typically this tension is 0.25 inches of water column, but may be elevated or lowered as the process needs. Isolators should be periodically leak tested to make sure their integrity and prevent escape of the decontamination agent. Leak testing can be carried out by several solutions like tension decay https://victorydirectory.com/listings1104711/5-easy-facts-about-aseptic-isolator-component-bags-described